the role of ghrelin and ghrelin receptors in polycystic ovary syndrome
نویسندگان
چکیده
background: polycystic ovary syndrome (pcos) is related to obesity and to major metabolic alterations including both insulin resistance and beta-cell dysfunction. ghrelin was identified as the endogenous ligand for the growth hormone secretagogue (ghs) receptor. the actions of ghrelin are carried out through interaction with specific receptor, named ghs-r. objective: in a case-control study, we compared the expression of ghrelin and ghs-rs mrna by quantitative real-time pcr method in studied groups in order to determine the role of ghrelin and ghs-rs in pathogenesis of pcos. materials & methods: follicular fluid samples were obtained at oocyte collection from 22 patients undergoing ivf-et as control and 11 patients were diagnosed as having pcos. total rna was extracted from isolated follicular fluid cells and 2�g rna was diluted and reverses transcribed using random primers and superscript ii. specific primers for the ghrelin, ghs-r1a and ghs-r1b were designed. samples were run in triplicate on an abi geneamp 5700 sequence detection system. they were subjected to 40 cycles of amplification under condition 92�c- 20s and 62�c-1min using 3�l diluted cdna (1:7), 10�l 2x sybr green, 3�l diluted cdna. ?-actin mrna was assayed and then normalized to total rna measurements for each sample. results: age, weight and resulting pregnancies did not vary between pcos and non-pcos patients, whereas the bmi and serum testosterone level of pcos were significantly higher than non-pcos patients. quantitative real-time rt-pcr showed that mrna for ghrelin and ghs-r 1b were detectable in follicular fluid cells from all patients. we failed to find mrna for ghs-r 1a in any of follicular fluid cells. there were no significant difference in ghrelin and ghs-r1b mrna expression levels between pcos and non-pcos groups. conclusion: our findings indicate that ghrelin and ghrelin receptors may not be considered risk factors for pathogenesis of pcos.
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عنوان ژورنال:
international journal of reproductive biomedicineجلد ۳، شماره ۲، صفحات ۶۸-۰
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